Device

Part:BBa_K185053:Design

Designed by: Shikun Zhao   Group: iGEM09_SJTU-BioX-Shanghai   (2009-10-18)


Promoter116+RBS34+LacI+Double terminator+Plac+RBS31+RelE+RBS30+Lon protease+Double terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2474
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 2474
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3187
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2474
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2474
    Illegal AgeI site found at 3650
    Illegal AgeI site found at 3734
    Illegal AgeI site found at 3932
    Illegal AgeI site found at 3965
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wish the expression of RelE and Lon protease can be controled by LacI.


Source

Promoter, RBS, lacI, plac, double terminator are come from 2009 iGEM biobrick. RelE and Lon protease come from E coli genome DNA

References